Trial sequential analysis (TSA) is a user-friendly software application which makes it easy for authors of systematic reviews and meta-analysis to apply a number of advanced sequential hypothesis testing techniques to their meta-analyses. TSA is free of charge. It is a Java-based software application which means that it runs on all operating systems that support Java (Windows, Mac OS, Linux and more), and it comes with a comprehensive manual suitable for systematic review authors with various levels of statistical background knowledge.

TSA is accompanied by the data converter application RM5 Converter. This application allows systematic review authors to convert their Review Manager v.5 meta-analysis to a format which can be read by the TSA application. RM5 Converter is also free of charge and Java-based. Instructions for the use of the RM5 Converter are provided in the accompanying manual.

TSA and RM5 Converter are distributed by the Copenhagen Trial Unit, Center for Clinical Intervention Research, Rigshospitalet, Copenhagen, Denmark.